產(chǎn)品詳情介紹
2×EasyTaq? PCR SuperMix (-dye)包含EasyTaq? DNA Polymerase、dNTPs和優(yōu)化的反應(yīng)緩沖液，濃度為2×。DNA擴(kuò)增時(shí)，只需加入模板、引物和水，使SuperMix溶液的濃度為1×即可進(jìn)行反應(yīng)。擴(kuò)增產(chǎn)物3' 端帶“A”堿基，2×EasyTaq? PCR SuperMix (-dye) 擴(kuò)增產(chǎn)物可直接克隆于pEASY?-T系列載體中；2×EasyTaq? PCR SuperMix (+dye) 擴(kuò)增產(chǎn)物可直接點(diǎn)樣電泳，如用于克隆，需純化去掉染料。其PCR產(chǎn)物不適用于聚丙烯酰胺凝膠電泳。
? 減少PCR擴(kuò)增操作時(shí)間。
? 避免因多步操作帶來的污染。
? 基因組DNA片段的擴(kuò)增 (≤4 kb)。
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2 Li C, Chen X, Wen R, et al. Immunocapture magnetic beads enhanced the LAMP-CRISPR/Cas12a method for the sensitive, specific, and visual detection of Campylobacter jejuni[J]. Biosensors, 2022.(IF 5.50)
3 Wang X, Liu C, Zhang S, et al. N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles[J]. Developmental cell, 2021.(IF 10.09)
4 Li D, Liu Y, Yi P, et al. RNA editing restricts hyperactive ciliary kinases[J]. Science, 2021.(IF 63.71)
5 Han D, Liu J, Chen C, et al. Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells[J]. Nature, 2019.(IF 64.8)
