產(chǎn)品詳情介紹
本試劑盒適用于從細(xì)胞和組織中提取總RNA，用TransZol Up裂解樣品，加入RNA Extraction Agent后，溶液分為無(wú)色水相和粉紅色有機(jī)相，RNA在水相中；用硅膠膜離心柱特異吸附水相中的RNA，與其它總RNA提取方法相比，既具有TransZol Up裂解能力強(qiáng)、提取量高，應(yīng)用范圍廣的優(yōu)點(diǎn)，又具有離心柱提取RNA純度高的優(yōu)點(diǎn)。
RNA提取效率高
使用TransGen ER501產(chǎn)品，分別以REA和氯仿作為抽提試劑，提取等量的Hela細(xì)胞的RNA，瓊脂糖凝膠電泳分析提取效果。結(jié)果表明， TransGen 產(chǎn)品RNA提取效率高，REA與氯仿提取效果相當(dāng)。
對(duì)下游實(shí)驗(yàn)無(wú)擴(kuò)增抑制
使用TransGen ER501產(chǎn)品，分別以REA和氯仿作為抽提試劑，提取Hela細(xì)胞的RNA，用TransGen一步法RT-PCR產(chǎn)品 (AT411)分析擴(kuò)增效果。結(jié)果表明，使用REA作為抽提試劑提取的RNA對(duì)下游PCR實(shí)驗(yàn)無(wú)擴(kuò)增抑制。
使用TransGen ER501產(chǎn)品，分別以氯仿和REA作為抽提試劑，提取Hela細(xì)胞、煙草、小鼠組織的RNA，分別用TransGen一步法RT-qPCR產(chǎn)品(AQ211)和兩步法RT-qPCR產(chǎn)品 (AUQ)分析擴(kuò)增效果。結(jié)果表明，使用REA作為抽提試劑提取的RNA對(duì)下游qPCR實(shí)驗(yàn)無(wú)擴(kuò)增抑制。
DNA殘留量低
使用TransGen ER501產(chǎn)品，分別以氯仿和REA作為抽提試劑 ，以提取的Hela細(xì)胞RNA為模板，人gDNA為引物，用染料法qPCR產(chǎn)品(AQ601)分析DNA殘留量。結(jié)果表明，使用REA作為抽提試劑提取的RNA中DNA殘留量低。
適用多物種RNA的提取
使用TransGen ER501產(chǎn)品，分別以REA和氯仿作為抽提試劑，提取293T細(xì)胞、小鼠肝組織、煙草的RNA，瓊脂糖凝膠電泳分析提取效果。結(jié)果表明，TransGen產(chǎn)品可用于提取多物種RNA。
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