產(chǎn)品詳情介紹
本產(chǎn)品以RNA為模板，在同一反應(yīng)體系中，合成第一鏈cDNA的同時去除RNA模板中殘留的基因組DNA。反應(yīng)結(jié)束后，只需在85℃加熱5秒鐘，即可同時失活TranScript? RT/RI與gDNA Remover。
? 在同一反應(yīng)體系中，同時完成反轉(zhuǎn)錄與基因組DNA的去除，操作簡便，降低污染機率。
? 產(chǎn)物用于qPCR：反轉(zhuǎn)錄15分鐘；產(chǎn)物用于PCR：反轉(zhuǎn)錄30分鐘。
? 反應(yīng)結(jié)束后，同時熱失活RT/RI與gDNA Remover。與傳統(tǒng)的用DNase I預(yù)處理RNA的方法相比，避免了處理后熱失活DNase I對RNA的損傷。
? 操作簡單。
?合成片段≤12 kb。
使用前請將各組分點甩離心
產(chǎn)品穩(wěn)定性
使用不同批次產(chǎn)品分別以人總RNA為模板，進行RT-PCR檢測，1.0%瓊脂糖凝膠電泳分析反轉(zhuǎn)錄效果。
使用不同批次產(chǎn)品分別以人100 ng總RNA、人100 ng總RNA+200 ng gDNA、200 ng gDNA為模板，進行RT-PCR檢測，1.0%瓊脂糖凝膠電泳分析模板DNA去除效果；qRT-PCR檢測18S RNA表達量。
與競品的比較
使用TransGen、Company TA和Company TH產(chǎn)品，分別以人100 ng總RNA、人100 ng總RNA+200 ng gDNA、200 ng gDNA為模板，進行RT-PCR檢測， 1.0%瓊脂糖凝膠電泳分析模板DNA去除效果；qRT-PCR檢測18S RNA表達量。
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