產(chǎn)品詳情介紹
本產(chǎn)品利用特殊的重組酶和同源重組的原理，可以將任意方法線性化后的載體和與其兩端具有15-25 bp重疊區(qū)域
的PCR片段定向重組，可以實(shí)現(xiàn)1-5個(gè)片段的高效無縫拼接。
? 快速：僅需要5~15分鐘反應(yīng)時(shí)間。
? 簡單：不受片段酶切位點(diǎn)的影響，無需對(duì)片段酶切。
? 高效：陽性率達(dá)95% 以上。
? 無縫：不引入額外的序列。
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2 Wan W, Dong H, Lai D H, et al. The Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartments[J]. Nature communications, 2023.(IF 17.69)
3 You L, Omollo E O, Yu C, et al. Structural basis for intrinsic transcription termination[J]. Nature, 2023.(IF 69.50)
4 Zhang H W, Huang K, Gu Z X, et al. A cryo-EM structure of KTF1-bound polymerase V transcription elongation complex[J]. Nature Communications, 2023.(IF 17.69)
5 Huang J, Yang L, Yang L, et al. Stigma receptors control intraspecies and interspecies barriers in Brassicaceae[J]. Nature, 2023(IF 64.80)
6 Tang J, Zhang G, Li F, et al. Two-Dimensional Protein Nanoarray as a Carrier of Sensing Elements for Gold-Based Immunosensing Systems[J]. Analytical Chemistry, 2022.(IF 8.00)
7 Wu M, Xu G, Han C, et al. lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription[J]. Science, 2021.(IF 63.71)
8 Chen Q, Hu T, Li X, et al. Phosphorylation of SWEET sucrose transporters regulates plant root: shoot ratio under drought[J]. Nature plants, 2022.(IF 15.79)
9 Liu R, Yang J, Yao J, et al. Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins[J]. Nature biotechnology, 2022.(IF 54.90)
10 Wu M, Xu G, Han C, et al. lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription[J]. Science, 2021.(IF 63.71)
11 Zeng Y H, Cai Z H, Zhu J M, et al. Two hierarchical LuxR-LuxI type quorum sensing systems in Novosphingobium activate microcystin degradation through transcriptional regulation of the mlr pathway[J]. Water Research, 2020.(IF 9.13)
